PXD013046
PXD013046 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii |
Description | The membrane-associated LonB protease is essential for viability in Haloferax volcanii, however, the cellular processes affected by this protease in archaea are unknown. In this study, the impact of a lon conditional mutation (down-regulation) on H. volcanii physiology was examined by comparing proteomes of parental and mutant cells using shotgun proteomics. A total of 1778 proteins were identified (44% of H. volcanii predicted proteome) and 142 changed significantly in amount (≥2 fold). Of these, 66 were augmented in response to Lon deficiency suggesting they could be Lon substrates. The “Lon subproteome” included soluble and predicted membrane proteins expected to participate in diverse cellular processes. The dramatic stabilization of phytoene synthase (57 fold) in concert with overpigmentation of lon mutant cells suggests that Lon controls carotenogenesis in H. volcanii. Several hypothetical proteins, which may reveal novel functions and/or be involved in adaptation to extreme environments, were notably increased (300 fold). This study, which represents the first proteome examination of a Lon deficient archaeal cell, shows that Lon has a strong impact on H. volcanii physiology evidencing the cellular processes controlled by this protease in Archaea. Additionally, this work provides a platform for the discovery of novel targets of Lon proteases. |
HostingRepository | jPOST |
AnnounceDate | 2020-03-11 |
AnnouncementXML | Submission_2022-09-18_03:05:35.236.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Micaela Cerletti |
SpeciesList | scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800; |
ModificationList | unknown modification; 2-pyrrolidone-5-carboxylic acid (Gln); L-methionine sulfoxide; deamidated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-03-11 10:49:27 | ID requested | |
1 | 2020-03-10 08:00:05 | announced | |
2 | 2021-02-04 16:58:26 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 03:05:35 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LonB protease, Quantitative shotgun proteomics, Protein substrates, Phytoene synthase, Archaea, Haloferax volcanii |
Contact List
Rosana De Castro | |
---|---|
lab head | |
Micaela Cerletti | |
contact affiliation | IIB-UNMdP |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000565/ |