PXD011703 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A chemical probe for Tudor domain protein SPIN1 to investigate chromatin functions |
Description | Lysine and arginine methylation are amongst the most frequent modifications on unstructured histone tails and in combination with other modifications provide the basis for a combinatorial 'chromatin or histone code'. Recognition of modified histone residues is accomplished in a specific manner by 'reader' domains that recognize chromatin modifications allowing to associate with specific effector complexes mediating chromatin functions. The methyl-lysine and methyl-arginine reader domain protein SPINDLIN1 (SPIN1) belongs to a family of 5 human genes, and has been identified as a putative oncogene and transcriptional co-activator containing three Tudor domains, able to mediate chromatin binding. Here we report on the discovery of the potent and selective bivalent Tudor domain inhibitor VinSPINIn* (see Footnote), which simultaneously engages Tudor domains 1 and 2 and effectively competes with chromatin binding. Inhibitor, chemoproteomic and knockdown studies in squamous cell carcinoma suggest an un-anticipated complexity of SPIN isoform mediated interactions in regulating cellular phenotypes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:33:38.090.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrew Lewis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-11-15 01:14:31 | ID requested | |
1 | 2022-02-23 09:06:06 | announced | |
⏵ 2 | 2024-10-22 05:33:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: affinity enrichment,Chemical Probe |
Contact List
Kilian Huber |
contact affiliation | Structural Genomics Consortium, University of Oxford, OX3 7DQ, Oxford, UK |
contact email | Kilian.Huber@sgc.ox.ac.uk |
lab head | |
Andrew Lewis |
contact affiliation | University of Oxford |
contact email | andrew.lewis@ndm.ox.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011703
- Label: PRIDE project
- Name: A chemical probe for Tudor domain protein SPIN1 to investigate chromatin functions