PXD011571

PXD011571 is an original dataset announced via ProteomeXchange.

Title	Selective autophagy degrades nuclear pore complexes
Description	Nuclear pore complexes (NPCs) are giant cylindrical structures embedded into the nuclear envelope and mediate nucleocytoplasmic exchange. NPC longevity has been linked to aging but since they appear to rarely turn over, the underlying mechanisms remain understudied. Here, we show that upon nitrogen starvation and genetic interference with NPC architecture, Nups are rapidly degraded in budding yeast. We demonstrate that NPC turnover involves 25 vacuolar proteases and the core autophagic machinery. Autophagic degradation is mediated by the cytoplasmically exposed Nup159 that serves as intrinsic cargo receptor and directly binds to the autophagic marker protein Atg8. The inducible manner of this mechanism might explain why NPCs are long-lived under specific conditions
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:49:45.309.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagarjuna Nagaraj
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-11-04 14:23:59	ID requested
1	2019-11-13 03:51:21	announced
⏵ 2	2024-10-22 04:49:45	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: autophagy, nuclear pore complexes

Boris Pfander
contact affiliation	Max Planck Institute of Biochemistry, DNA Replication and Genome Integrity, 82152 Martinsried, Germany
contact email	bpfander@biochem.mpg.de
lab head
Nagarjuna Nagaraj
contact affiliation	MaxPlanck Institute for Biochemistry, Core facility
contact email	nagaraj@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD011571

PRIDE project URI

• PRIDE
  1. PXD011571
     1. Label: PRIDE project
     2. Name: Selective autophagy degrades nuclear pore complexes