PXD011251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AML1-ETO induced leukemia stem cell self-renewal depends on Plcg1 |
Description | High-resolution proteomic analysis of acute myeloid leukemia (AML) stem cells identified phospholipase C- and Ca++-signaling pathways to be differentially regulated in AML1-ETO (AE) driven leukemia. Phospholipase C gamma 1 (Plcg1) could be identified as a direct target of the AE fusion. Genetic Plcg1 inactivation abrogated disease initiation by AE, reduced intracellular Ca++-release and inhibited AE-driven self-renewal programs. In AE-induced leukemia, Plcg1 deletion significantly reduced disease penetrance, number of leukemia stem cells and abrogated leukemia development in secondary recipient hosts. In human AE-positive leukemic cells inactivation of Plcg1 reduced colony formation and AML development in vivo. In contrast, Plcg1 was dispensable for maintenance of murine and human hematopoietic stem- and progenitor cells (HSPCs). Pharmacologic inhibition of Ca++-signaling downstream of Plcg1 resulted in impaired proliferation and self-renewal capacity in AE-driven AML. Thus, the Plcg1 pathway represents a novel specific vulnerability of AE-driven leukemia and poses an important new therapeutic target. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:30:51.675.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joanna Kirkpatrick |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-10-02 05:38:57 | ID requested | |
1 | 2022-01-12 02:54:56 | announced | |
⏵ 2 | 2024-10-22 05:30:52 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: acute myeloid leukemia, stem cells |
Contact List
Florian H. Heidel |
contact affiliation | Professor for Stem Cell Aging Faculty of Medicine Friedrich-Schiller-University Jena Erlanger Allee, 07747 Jena; Germany |
contact email | florian.heidel@leibniz-fli.de |
lab head | |
Joanna Kirkpatrick |
contact affiliation | The Francis Crick Institute |
contact email | joanna.kirkpatrick@leibniz-fli.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011251
- Label: PRIDE project
- Name: AML1-ETO induced leukemia stem cell self-renewal depends on Plcg1