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PXD011221

PXD011221 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImproved SILAC Method for Double Labeling of Bacterial Proteome
DescriptionStable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quantification accuracy, limiting the application of SILAC in bacterial proteomics to auxotrophic bacteria or single labeling with lysine. In this study, we found that adding high concentrations of isotope-labeled (heavy) and natural (light) amino acids into SILAC minimal medium can efficiently inhibit the complicated amino acid conversions between each other. This simple and straightforward strategy facilitated the full incorporation of amino acids into the bacterial proteome with good accuracy. The high labeling efficiency can be reached in different bacteria by slightly modifying the supplement of amino acids in culture media, promoting the widespread application of SILAC technique in bacterial proteomics.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:57.997.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYi shuhong
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListcarbamoylated residue; monohydroxylated residue; acetylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-28 07:24:52ID requested
12022-03-02 02:51:03announced
22023-11-14 08:49:58announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: proteomics, double labeling, bacteria,SILAC
Contact List
Xuesong Sun
contact affiliationKey Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
contact emailtsunxs@jnu.edu.cn
lab head
Yi shuhong
contact affiliationJinan University
contact emailelizabethyi705@yahoo.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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