PXD011221 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Improved SILAC Method for Double Labeling of Bacterial Proteome |
Description | Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with the advantages of reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quantification accuracy, limiting the application of SILAC in bacterial proteomics to auxotrophic bacteria or single labeling with lysine. In this study, we found that adding high concentrations of isotope-labeled (heavy) and natural (light) amino acids into SILAC minimal medium can efficiently inhibit the complicated amino acid conversions between each other. This simple and straightforward strategy facilitated the full incorporation of amino acids into the bacterial proteome with good accuracy. The high labeling efficiency can be reached in different bacteria by slightly modifying the supplement of amino acids in culture media, promoting the widespread application of SILAC technique in bacterial proteomics. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:49:57.997.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yi shuhong |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | carbamoylated residue; monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-28 07:24:52 | ID requested | |
1 | 2022-03-02 02:51:03 | announced | |
⏵ 2 | 2023-11-14 08:49:58 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, double labeling, bacteria,SILAC |
Contact List
Xuesong Sun |
contact affiliation | Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China |
contact email | tsunxs@jnu.edu.cn |
lab head | |
Yi shuhong |
contact affiliation | Jinan University |
contact email | elizabethyi705@yahoo.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD011221
- Label: PRIDE project
- Name: Improved SILAC Method for Double Labeling of Bacterial Proteome