PXD011211 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly |
Description | TFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique subunit composition exist and several TFIID subunits are shared with other complexes, conveying intricate cellular decision making to control subunit allocation and functional assembly of this essential transcription factor. However, the underlying molecular mechanisms remain poorly understood. Here, we used quantitative proteomics to examine TFIID submodules and assembly mechanisms in human cells. Structural and mutational analysis of the cytoplasmic TAF5/TAF6/TAF9 submodule identified novel interactions crucial for TFIID integrity, and for allocating TAF9 to TFIID or the SAGA co-activator complex. We discover a key checkpoint function for the chaperonin CCT, which specifically associates with nascent TAF5 for subsequent handover to TAF6/TAF9 and ultimate holo-TFIID formation. Our findings illustrate at the molecular level how multisubunit complexes are crafted in the cell, involving checkpoint decisions facilitated by a chaperone machine. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:49:36.624.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Simona Antonova |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-09-27 11:32:02 | ID requested | |
1 | 2022-03-02 07:12:58 | announced | |
⏵ 2 | 2023-11-14 08:49:37 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proteomics , crystal structure, TFIID, chaperonin CCT |
Contact List
Marc Timmers |
contact affiliation | Professor of Medical Epigenetics German Cancer Consortium (DKTK), partner site Freiburg German Cancer Research Center (DKFZ) Foundation under Public Law c/o Zentrale Klinische Forschung (ZKF) University Medical Center Freiburg Breisacher Str. 66, 79106 Freiburg, Germany |
contact email | m.timmers@dkfz-heidelberg.de |
lab head | |
Simona Antonova |
contact affiliation | RMCU, University Medical Center, Utrecht |
contact email | s.v.antonova-2@umcutrecht.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD011211
- Label: PRIDE project
- Name: Chaperonin CCT Checkpoint Function in Basal Transcription Factor TFIID Assembly