PXD011143

PXD011143 is an original dataset announced via ProteomeXchange.

Title	Proteome Profiling of PBMC-originated, iPSC-derived Lentoid Bodies at differentiation day 25
Description	Differentiation of pluripotent cells to generate lentoid bodies is important for the understanding of the lens development and investigating the processes critical for lens morphogenesis. This Study was initiated to investigate a comprehensive proteome profiling of the peripheral blood mononuclear cell (PBMC)-originated, induced pluripotent stem cell (iPSC)-derived lentoid bodies through mass spectrometry-based protein sequencing. Briefly, a small aliquot of blood sample was ascertained to collect PBMCs that were reprogrammed to iPSCs using the Sendai-virus delivery system. The PBMC-originated, iPSCs were differentiated into lentoid bodies employing the “fried egg” method using feeder-free conditions. The quantitative real-time PCR (qRT-PCR) confirmed the expression of lens-associated markers, which exhibited at least an order magnitude increased expression in lentoid bodies at differentiation day 25. Subsequently, the total cellular protein was extracted from lentoid bodies at day 25, digested with trypsin, fractionated into 24 fractions and subjected to an mass spectrometry-based label-free quantitative proteomics. mass spectrometry-based proteome profiling revealed 9,473 proteins in iPSC-derived lentoid bodies at differentiation day 25. In here, we report a comprehensive proteome of PBMC-originated, iPSC-derived lentoid bodies at day 25, which will help in better understanding processes critical for the development of the ocular lens.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:43.930.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	OM Genetics
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-19 03:20:38	ID requested
1	2022-03-01 09:52:03	announced
⏵ 2	2024-10-22 05:34:44	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mass-spectrometry, iPSC-derived Lentoid Bodies, Proteome,PBMC

S. Amer Riazuddin
contact affiliation	The Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 USA
contact email	riazuddin@jhmi.edu
lab head
OM Genetics
contact affiliation	Johns Hopkins Wilmer Eye Institute
contact email	omgenetics@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011143
     1. Label: PRIDE project
     2. Name: Proteome Profiling of PBMC-originated, iPSC-derived Lentoid Bodies at differentiation day 25