PXD010817

PXD010817 is an original dataset announced via ProteomeXchange.

Title	Detergent-resistant microdomains in Saprolegnia parasitica
Description	Oomycete cells are surrounded by a polysaccharide rich cell wall matrix that, in addition to being essential for cell growth, also functions as protective ”armour”. It follows, that the enzymes responsible for the synthesis of the cell wall provide potential targets for disease management. Interestingly, the oomycete cell wall enzymes are predicted to be plasma membrane proteins. In this project, we used a quantitative (iTRAQ) mass spectrometry-based proteomics approach to characterize the plasma membrane proteome of the hyphal cells of S. parasitica, providing the first complete plasma membrane proteome of an oomycete species. Of significance, is the identification of proteins enriched in functional microdomains (Detergent-Resistant Microdomains; DRMs). In silico analysis showed that DRM-enriched proteins are mainly involved in both molecular transport and β-1,3-glucan synthesis, potentially contributing to post infection pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:39:04.109.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vaibhav Srivastava
SpeciesList	scientific name: Saprolegnia parasitica; NCBI TaxID: 101203;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	Q-Tof Ultima

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-20 02:08:00	ID requested
⏵ 1	2024-10-08 11:39:04	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: mass spectrometry,Saprolegnia parasitica, detergent-resistant microdomains, iTRAQ, plasma membrane

Vaibhav Srivastava
contact affiliation	Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health AlbaNova University Center, KTH Royal Institute of Technology
contact email	vasri@kth.se
lab head
Vaibhav Srivastava
contact affiliation	Biotechnology
contact email	vasri@kth.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010817
     1. Label: PRIDE project
     2. Name: Detergent-resistant microdomains in Saprolegnia parasitica