<<< Full experiment listing

PXD010346

PXD010346 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics reveals localization of cuticular proteins in Anopheles gambiae
DescriptionAnopheles gambiae devotes over 2% of its protein coding genes to its 298 structural cuticular proteins (CPs). This paper provides new LC-MS/MS data on two adult structures, probosces and palps, as well as three larval samples: 4th instar larvae, just their terminal segment, and a preparation enriched in their tracheae. These data were combined with our previously published results of proteins from four other adult structures: whole adults, two preparations chosen for their relatively clean cuticle, the larval head capsules left behind after ecdysis, and the pupal cuticles left behind after adult eclosion. Peptides from 28 CPs were recovered in all adult structures; 24 CPs were identified for the first time, 6 were members of the TWDL family. Most newly identified proteins came from the larval sources. Based solely on peptide recovery, from our data and from other investigators mostly available on VectorBase, there were only 5 CPs that were restricted to a single structure. More were restricted to a single metamorphic stage, 14 in larvae, 0 in pupa and 31 in adults. Expression data from our earlier RT-qPCR studies reduces these numbers. Charting restriction of CPs to stage or structure is a step forward in establishing their specific roles.
HostingRepositoryMassIVE
AnnounceDate2018-07-09
AnnouncementXMLSubmission_2018-07-09_21:09:04.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMajors Badgett
SpeciesList scientific name: Anopheles gambiae; common name: African malaria mosquito; NCBI TaxID: 7165;
ModificationListCarbamidomethyl; Oxidation; Deamidated
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-09 19:58:41ID requested
12018-07-09 21:09:04announced
Publication List
no publication
Keyword List
submitter keyword: Probosces, Palps, Trachea, Larvae
Contact List
Dr. Judith H. Willis
contact affiliationUniversity of Georgia
contact emailjhwillis@uga.edu
lab head
Majors Badgett
contact affiliationUniversity of Georgia
contact emailmajorsbadgett@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000082587