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PXD010170

PXD010170 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative phosphoproteomic analysis of barley grains
DescriptionSeed dormancy is important trait for plant to grow in favorable condition. It is strongly maintained in freshly harvested seed, but impaired in after-ripened seeds. In dormancy regulation, phytohormone abscisic acid (ABA) has essential role. It is transduced thorough protein phosphorylation and occur induction and maintenance of dormancy. To understand the role of ABA during after-ripening, ABA responsive phosphoproteins should be understood. Phosphoproteomic analysis was carried out by using following process. Isolated barley seed embryo was used for protein extraction. Trypsin digestion and phosphopeptide enrichment by HAMMOC method were performed, then purified peptide sample was analyzed by TripleTOF5600 (AB-SCIEX).
HostingRepositoryjPOST
AnnounceDate2019-06-20
AnnouncementXMLSubmission_2022-09-18_02:50:55.965.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterShinnosuke ISHIKAWA
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-06-20 00:16:02ID requested
12019-06-19 17:12:19announced
22021-02-04 16:52:11announced2021-02-04: Updated FTP location.
32022-09-18 02:50:56announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphoproteome, barley, after-ripening
Contact List
Shinnosuke ISHIKAWA
lab head
Shinnosuke ISHIKAWA
contact affiliationTokyo University of Agriculture and Technology
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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