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PXD009547

PXD009547 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA novel differential ion mobility interface expands the depth of proteome coverage and the sensitivity of multiplex proteomic measurements
DescriptionWe present a new high field asymmetric waveform ion mobility spectrometry (FAIMS) interface that can be coupled to the Orbitrap Tribrid mass spectrometers. The interface provides several advantages over previous generations of FAIMS devices including ease of operation, robustness, and high ion transmission. Replicate LC-FAIMS-MS/MS analyses (N=100) of protein digests showed stable ion current over extended time periods with uniform peptide identification on more than 10,000 distinct peptides. For complex tryptic digest analyses, the coupling of FAIMS to LC-MS/MS enabled a 30% gain in unique peptide identification compared to non FAIMS experiments. Improvement in sensitivity facilitated the identification of low abundance peptides, and extended the limit of detection by almost an order of magnitude. The reduction in chimeric MS/MS spectra using FAIMS also improved the precision and the number of quantifiable peptides when using isobaric labeling with tandem mass tag (TMT) 10-plex reagent. We compared quantitative proteomic measurements for LC-MS/MS analyses performed using synchronous precursor selection (SPS) and LC-FAIMS-MS/MS to profile the temporal changes in protein abundance of HEK293 cells following heat shock for periods up to 9h. FAIMS provided 2.5-fold increase in the number of quantifiable peptides compared to non-FAIMS. Altogether, the enhancement in ion transmission and duty cycle of the new FAIMS interface extended the depth and comprehensiveness of proteomic analyses and improved the precision of quantitative measurements.
HostingRepositoryMassIVE
AnnounceDate2018-07-31
AnnouncementXMLSubmission_2018-07-31_09:38:08.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancis McManus
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListPhospho; Deamidated; Oxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-04-19 09:02:57ID requested
12018-07-31 09:38:09announced
Publication List
no publication
Keyword List
submitter keyword: High field asymmetric waveform ion mobility spectrometry (FAIMS), quantitative proteomics, isobaric peptide labeling, tandem mass tag (TMT), heat shock
Contact List
Pierre Thibault
contact affiliationUniversity of Montreal
contact emailpierre.thibault@umontreal.ca
lab head
Francis McManus
contact affiliationUniversity of Montreal
contact emailfrancis.mcmanus@umontreal.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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