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PXD008705

PXD008705 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiPIUS_Fbxw7
DescriptionAlthough elucidation of enzyme–substrate relations is fundamental to the advancement of biology, universal approaches to the identification of substrates for a given enzyme have not been established. It is especially difficult to identify substrates for ubiquitin ligases, given that most such substrates are immediately ubiquitylated and degraded as a result of their association with the enzyme. We here describe the development of a new approach, DiPIUS (differential proteomics-based identification of ubiquitylation substrates), to the discovery of substrates for ubiquitin ligases. We applied DiPIUS to Fbxw7α, and identified candidate substrates including previously known targets. DiPIUS is thus a powerful tool for unbiased and comprehensive screening for substrates of ubiquitin ligases.
HostingRepositoryjPOST
AnnounceDate2018-03-06
AnnouncementXMLSubmission_2022-09-18_03:39:01.838.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKanae Yumimoto
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; 2-pyrrolidone-5-carboxylic acid (Gln)
InstrumentLTQ
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-01-17 17:14:06ID requested
12018-03-06 00:35:30announced
22018-09-19 00:45:36announcedUpdated CV, FTP location and Modification list.
32021-02-04 17:00:45announced2021-02-04: Updated FTP location.
42022-09-18 03:39:02announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: substrates, Fbxw7, ubiquitin ligase
Contact List
Keiichi I. Nakayama
lab head
Kanae Yumimoto
contact affiliationKyushu University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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