PXD008313

PXD008313 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen
Description	The absolute amount of plastocyanin (PC), ferredoxin-NADP+-oxidoreductase (FNR), hydrogenase (HYDA1) and ferredoxin 5 (FDX5) were quantified in aerobic and anaerobic Chlamydomonas reinhardtii whole cells using purified (recombinant) proteins as internal standard in a mass spectrometric approach. Quantified protein amounts were related to the estimated amount of PSI. The ratios of PC to FNR to HYDA1 to FDX5 in aerobic cells were determined to be 2.1 : 1.8 : 0.005 : 0. In anaerobic cells, the ratios changed to 1.6 : 1.8 : 0.026 : 0.04 (PC : FNR : HYDA1 : Fdx5). Employing sodium dithionite and methyl viologen as electron donors the specific activity of hydrogenase in whole cells was calculated to be 382 ±96.5 μmolH2/min/mg. Importantly, these data demonstrate for competition of electrons from FDX1, HYDA1 is about 70-fold less abundant than its competitor FNR. Effective in vivo hydrogen production points to a channeling mechanism for FDX to HYDA1 electron transfer.
HostingRepository	PRIDE
AnnounceDate	2022-02-23
AnnouncementXML	Submission_2022-02-23_08:42:19.342.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Denitsa Nikolova
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-28 05:58:49	ID requested
⏵ 1	2022-02-23 08:42:19	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: plastocyanin, hydrogenase, ferredoxin-NADP+-oxidoreductase, absolute quantification, ferredoxin 5, Chlamydomonas reinhardtii

Michael Hippler
contact affiliation	University Münster, Institute for Biology and Biotechnology of Plants
contact email	mhippler@uni-muenster.de
lab head
Denitsa Nikolova
contact affiliation	University of Münster
contact email	dennitsa.nikolova@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD008313

PRIDE project URI

• PRIDE
  1. PXD008313
     1. Label: PRIDE project
     2. Name: Absolute quantification of selected photosynthetic electron transfer proteins in Chlamydomonas reinhardtii in the presence and absence of oxygen