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PXD008214

PXD008214 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHost-pathogen dynamics through secretome analysis of stimulated macrophages
DescriptionThe Toll-like receptors (TLRs) recognize different pathogen associated molecular patterns (PAMPs) and promote MyD88 dependent and independent responses. Previously, we have reported the discovery of the temporal changes in signaling cascades of macrophage proteome and secretome post-stimulation with different PAMPs. Here we present strategies to profile the secretome of TLR2-, TLR4, and TLR7- stimulated macrophages using whole pathogens were developed. Stable isotope labeling with amino acids in cell culture of macrophages was integrated with whole pathogen macrophage stimulation and subsequent targeted proteomics to quantify cytokines, chemokines, and transcription factors.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:34:12.824.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAleksandra Nita-Lazar
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-11-15 01:23:20ID requested
12022-02-28 03:52:38announced
22024-10-22 05:34:13announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TLR4, macrophage, TLR2, TLR7,Secretome, targeted proteomics, LC-MS/MS
Contact List
Aleksandra Nita-Lazar
contact affiliationInvestigator Chief, Cellular Networks Proteomics Unit Laboratory of Immune System Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bldg. 4 Rm. 101 4 Memorial Dr Bethesda MD 20892-1892
contact emailnitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliationNIAID
contact emailnitalazarau@niaid.nih.gov
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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