PXD006228

PXD006228 is an original dataset announced via ProteomeXchange.

Title	Distribution of Cuticular Proteins in Different Structures of Adult Anopheles Gambiae
Description	Anopheles gambiae devotes over 2% (295) of its protein coding genes to structural cuticular proteins (CPs) that have been classified into 13 different families plus ten low complexity proteins not assigned to families. Small groups of genes code for identical proteins reducing the total number of unique cuticular proteins to 282. Is the large number because different structures utilize different CPs, or are all of the genes widely expressed? We used LC-MS/MS to learn how many products of these genes were found in five adult structures: Johnston's organs, the remainder of the male antennae, eye lenses, legs, and wings. Data were analyzed against both the entire proteome and a smaller database of just CPs. We recovered unique peptides for 97 CPs and shared peptides for another 35. Members of 11 of the 13 families were recovered as well as some unclassified. Only 11 CPs were present exclusively in only one structure while 43 CPs were recovered from all five structures. A quantitative analysis, using normalized spectral counts, revealed that only a few CPs were abundant in each structure. When the MS/MS data were run against the entire proteome, the majority of the top hits were to CPs, but peptides were recovered from an additional 467 proteins. CP peptides were frequently recovered from chitin-binding domains, confirming that protein-chitin interactions are not mediated by covalent bonds. Comparison with three other MS/MS analyses of cuticles or cuticle-rich structures augmented the current analysis. Our findings provide new insights into the composition of different mosquito structures and reveal the complexity of selection and utilization of genes coding for structural cuticular proteins.
HostingRepository	MassIVE
AnnounceDate	2017-04-03
AnnouncementXML	Submission_2017-04-03_10:41:54.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Majors Badgett
SpeciesList	scientific name: Anopheles gambiae; common name: African malaria mosquito; NCBI TaxID: 7165;
ModificationList	Oxidation; Deamidated; Carbamidomethyl
Instrument	LTQ; Orbitrap Fusion; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-03-31 15:17:48	ID requested
⏵ 1	2017-04-03 10:41:55	announced

Dataset with its publication pending

submitter keyword: Anopheles Gambiae, Structures, LC-MS/MS, Cuticlar Protein

Dr. Judith H. Willis
contact affiliation	University of Georgia
contact email	jhwillis@uga.edu
lab head
Majors Badgett
contact affiliation	University of Georgia
contact email	majorsbadgett@gmail.com
dataset submitter

MassIVE dataset URI

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080830