PXD005758 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A method for spatially and temporally resolved protein network interrogation in living cells |
Description | Cells operate through protein interaction networks organized in space and in time, but current methods to interrogate such biology typically resolve only one of these dimensions. Here, we describe a method to resolve both dimensions simultaneously using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to label organelle proteomes with high temporal resolution, but its spatial resolution is generally thought to be limited because of its larger labeling radius. We describe an analytical pipeline, based on quantitative proteomics using spatial references, which overcomes this barrier. As proof-of-principle, we apply the methodology to interrogate protein interaction networks of G protein coupled receptors both temporally and spatially. Using this approach, we identify known binding partners as well as novel receptor regulators. Validating the methodology as a discovery tool, we demonstrate that two of these components drive ubiquitin-linked down-regulation of opioid receptors. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:32:20.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ruth Huttenhain |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-01-21 07:53:50 | ID requested | |
1 | 2017-03-03 02:04:43 | announced | |
⏵ 2 | 2024-10-22 04:32:20 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: APEX, proximity biotinylation,GPCR, protein-protein interactions |
Contact List
Ruth Huttenhain |
contact affiliation | Department of Molecular & Cellular Pharmacology UCSF, San Francisco, CA |
contact email | ruth.huttenhain@ucsf.edu |
lab head | |
Ruth Huttenhain |
contact affiliation | UCSF |
contact email | ruth.huttenhain@ucsf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005758 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005758
- Label: PRIDE project
- Name: A method for spatially and temporally resolved protein network interrogation in living cells