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PXD005758

PXD005758 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA method for spatially and temporally resolved protein network interrogation in living cells
DescriptionCells operate through protein interaction networks organized in space and in time, but current methods to interrogate such biology typically resolve only one of these dimensions. Here, we describe a method to resolve both dimensions simultaneously using proximity labeling mediated by engineered ascorbic acid peroxidase (APEX). APEX has been used to label organelle proteomes with high temporal resolution, but its spatial resolution is generally thought to be limited because of its larger labeling radius. We describe an analytical pipeline, based on quantitative proteomics using spatial references, which overcomes this barrier. As proof-of-principle, we apply the methodology to interrogate protein interaction networks of G protein coupled receptors both temporally and spatially. Using this approach, we identify known binding partners as well as novel receptor regulators. Validating the methodology as a discovery tool, we demonstrate that two of these components drive ubiquitin-linked down-regulation of opioid receptors.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:32:20.180.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRuth Huttenhain
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-01-21 07:53:50ID requested
12017-03-03 02:04:43announced
22024-10-22 04:32:20announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
curator keyword: Technical, Biological
submitter keyword: APEX, proximity biotinylation,GPCR, protein-protein interactions
Contact List
Ruth Huttenhain
contact affiliationDepartment of Molecular & Cellular Pharmacology UCSF, San Francisco, CA
contact emailruth.huttenhain@ucsf.edu
lab head
Ruth Huttenhain
contact affiliationUCSF
contact emailruth.huttenhain@ucsf.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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