PXD005222 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tapered-tip Capillary Electrophoresis Nano-electrospray Ionization Mass Spectrometry for Ultrasensitive Proteomics: The Mouse Cortex |
Description | We have enabled ultrasensitive proteomics of limited protein digests by developing a tapered-tip nanoelectrospray ionization (nanoESI) interface for microanalytical capillary electrophoresis (CE) high resolution mass spectrometry (HRMS). CE-nanoESI-HRMS allowed us to detect 260 zmol angiotensin II (lower limit of detection) and identify 1 pg of bovine serum albumin and cytochrome c. A 1 ng protein digest from the mouse posterior cortex yielded 217 nonredundant protein groups, including many gene products that are used as classical neuronal markers. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:32:54.364.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Nemes |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-10-25 02:25:12 | ID requested | |
1 | 2022-02-18 15:56:22 | announced | |
⏵ 2 | 2024-10-22 05:32:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: liquid chromatography, brain,Capillary electrophoresis, mass spectrometry, bottom-up proteomics, mouse, nanoelectrospray ionization, Impact HD |
Contact List
Peter Nemes |
contact affiliation | The George Washington University |
contact email | Petern@gwu.edu |
lab head | |
Peter Nemes |
contact affiliation | University of Maryland, College Park |
contact email | nemes@umd.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005222
- Label: PRIDE project
- Name: Tapered-tip Capillary Electrophoresis Nano-electrospray Ionization Mass Spectrometry for Ultrasensitive Proteomics: The Mouse Cortex