PXD004590 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterisation of the human hemostatic O-glycoproteome |
Description | The hemostatic system comprises platelet aggregation, coagulation and fibrinolysis, and is critical to the maintenance of vascular integrity. Multiple studies indicate that glycans play an important role in maintaining the hemostatic system, however, most investigations have focused on N-glycans and little is known about the location and function of O-glycans. Here we performed the first systematic analysis of hemostatic O-glycosylation using lectin affinity chromatography coupled to LC-MS/MS to determine the precise location of O-glycans in human plasma, platelets, and endothelial cells. We identified the hitherto largest O-glycoproteome from native tissue, demonstrating that O-glycosylation is a ubiquitous modification of hemostatic proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:49:10.429.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sarah King |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; Hex-HexNAc; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-07-18 02:51:31 | ID requested | |
1 | 2017-03-15 06:02:00 | announced | |
2 | 2017-08-06 23:57:28 | announced | Updated project metadata. |
⏵ 3 | 2023-11-14 08:49:10 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biomedical |
submitter keyword: hemostatic, LC-MSMS, plasma, serum, orbitrap, endothelium,human, endothelial, platelet |
Contact List
Hans Wandall |
contact affiliation | University of Copenhagen Faculty Of Health and Medical Sciences Centre for Glycomics Department of Cellular and Molecular Medicine Building 24.6.38 Nørre Alle 20, DK-2200 Copenhagen N Denmark |
contact email | hhw@sund.ku.dk |
lab head | |
Sarah King |
contact affiliation | Copenhagen University |
contact email | sarahki@sund.ku.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD004590 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD004590
- Label: PRIDE project
- Name: Characterisation of the human hemostatic O-glycoproteome