PXD003834

PXD003834 is an original dataset announced via ProteomeXchange.

Title	Role of CLRC complex in telomeric assembly of shelterin proteins
Description	Heterochromatin represents a specific structural organization of chromatin that is essential for cellular development and genome stability. In S. pombe, all members of the Cul4-Rik1Raf1/2 E3 ubiquitin ligase complex are critical for heterochromatin integrity. This key complex assembles with Clr4 (the sole H3K9 methyltransferase) to form the CLRC, which is required for silencing of the three main heterochromatic regions (centromere, telomeres and mating locus). Although much effort has been invested in understanding the function of this key complex, the ubiquitylation substrate(s) of CLRC remain unknown. To overcome the challenge of transient interactions between substrate and ubiquitin ligase in vivo, we have established the Ubiquitin Ligase Trapping method in S. pombe (Mark KG et al., Mol. Cell 2014). This approach is based on fusing a ubiquitin associated domain (UBA) to the E3 ligase by a flexible linker in order to trap the ubiquitylated substrate allowing the analysis of E3-associated proteins by mass spectrometry. We have been able to reproducibly detect chromatin related proteins associated with CRLC and to validate their interactions. Interestingly, among these candidates we identified the telomeric shelterin complex as a new interactor of CLRC.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:08:27.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Schizosaccharomyces pombe DM3650; NCBI TaxID: 614666;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-03-22 02:37:47	ID requested
1	2019-02-19 02:12:06	announced
⏵ 2	2024-10-22 04:08:27	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: S. pombe, telomeres, heterochromatin

Prof Axel Imhof
contact affiliation	Zentrallabor für Proteinanalytik Room NC 01.044 LS Molekularbiologie BioMedical Center Ludwig-Maximilians-Universität Großhadernerstr. 9 82152 Planegg-Martinsried
contact email	imhof@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD003834

PRIDE project URI

• PRIDE
  1. PXD003834
     1. Label: PRIDE project
     2. Name: Role of CLRC complex in telomeric assembly of shelterin proteins