PXD003344
PXD003344 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry |
Description | In this study, targeted parallel reaction monitoring assays for phosphopeptides were configured by mining data from large-scale experiments. The capabilities of the method were assessed by performing several benchmarking experiments. The accuracy of retention time prediction using a large-scale database was assessed by using BSA peptides or human phosphopeptides to predict the retention time of distinct peptides in a subsequent run. Data-driven phosphopeptide sequence and charge state was compared to heuristics-based selection using unscheduled PRM assays. Phosphoproteome analysis was performed in technical quadruplicate using parallel reaction monitoring, data-independent acquisition, and data-dependent acquisition. A label-free quantitative parallel reaction monitoring experiment was performed on phosphopeptides enriched from cells stimulated -/+ IGF-1 (n=6). |
HostingRepository | MassIVE |
AnnounceDate | 2016-03-27 |
AnnouncementXML | Submission_2016-03-27_23:43:38.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Lawrence |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Phospho |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2015-12-14 15:56:33 | ID requested | |
⏵ 1 | 2016-03-27 23:43:39 | announced |
Publication List
Robert T. Lawrence, Brian C. Searle, Ariadna Llovet, Judit Villén. Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry. Nature Methods. 2016 Mar. doi: 10.1038/nmeth.3811. |
Keyword List
submitter keyword: phosphoproteomics, targeted analysis, PRM, DDA, DIA |
Contact List
Judit Villén | |
---|---|
lab head | |
Robert Lawrence | |
contact affiliation | University of Washington |
contact email | roblaw@uw.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079423 |