PXD002791

PXD002791 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Ubiquitin-activated interaction traps (UBAITs) identify E3 ligase binding partners
Description	We describe a new class of reagents for identifying substrates, adaptors, and regulators of HECT and RING E3s. UBAITs (Ubiquitin-Activated Interaction Traps) are E3-ubiquitin fusion proteins, and in an E1- and E2-dependent manner, the C-terminal ubiquitin moiety forms an amide linkage to proteins that interact with the E3, enabling covalent co-purification of the E3 with partner proteins. UBAITs were made for both HECT (Rsp5, Itch) and RING (Psh1, RNF126, RNF168) E3s. For HECT E3s, substrate trapping occurred in vitro either through an E3 thioester-linked lariat intermediate or an E2 thioester intermediate, and both WT and active site mutant UBAITs trapped known interacting proteins in yeast and human cells. Yeast Psh1 and human RNF126 and RNF168 UBAITs also trapped known interacting proteins when expressed in cells.
HostingRepository	MassIVE
AnnounceDate	2015-08-25
AnnouncementXML	Submission_2015-08-25_08:55:09.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andre Bui
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationList	ubiquitination signature dipeptidyl lysine; Carbamidomethyl; Oxidation
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2015-08-25 08:35:33	ID requested
⏵ 1	2015-08-25 08:55:09	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: UBAITs, ubiquitin, ubiquitin ligases, human 293T, Saccharomyces cerevisiae

submitter keyword: UBAITs, ubiquitin, ubiquitin ligases, human 293T, Saccharomyces cerevisiae

Contact List

Jon Huibregtse
lab head
Andre Bui
contact affiliation	University of Texas at Austin
contact email	pmaf@austin.utexas.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000079265