PXD002096 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The influence of simulated microgravity on the proteome of Daphnia magna |
Description | Background/Objectives: The waterflea Daphnia is an interesting candidate for biore- generative life support systems (BLSS). These animals are particularly promising be- cause of their central role in the limnic food web and its mode of reproduction. How- ever, the response of Daphnia to altered gravity conditions has to be investigated, especially on the molecular level, to evaluate the suitability of Daphnia for BLSS in space. Methods: In this study, we applied a proteomic approach to identify key proteins and pathways involved in the response of Daphnia to simulated microgravity gener- ated by a 2D-clinostat. We analysed 5 biological replicates using 2D-DIGE proteomic analysis. Results: We identified 109 protein spots differing in intensity (p < 0.05). Substan- tial fractions of these proteins are involved in actin microfilament organisation, in- dicating the disruption of cytoskeletal structures during clinorotation. Furthermore, proteins involved in protein folding were identified, suggesting altered gravity in- duced break-down of protein structures in general. In addition, simulated micro- gravity increased the abundance of energy metabolism related proteins, indicating an enhanced energy demand of Daphnia. Conclusion: The affected biological processes were also described in other studies using different organisms and systems either aiming to simulate microgravity con- ditions or providing real microgravity conditions. Moreover, most of the Daphnia protein sequences are well conserved throughout taxa, indicating that the response to altered gravity conditions in Daphnia follows a general concept. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:48:50.223.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kathrin Otte |
SpeciesList | scientific name: Daphnia magna; NCBI TaxID: 35525; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-04-22 00:54:51 | ID requested | |
1 | 2015-09-09 05:16:07 | announced | |
2 | 2016-06-01 03:54:17 | announced | Updated publication reference for DOI(s): http://dx.doi.org/10.1038/npjmgrav.2015.16. |
3 | 2017-08-06 23:56:42 | announced | Updated project metadata. |
⏵ 4 | 2023-11-14 08:48:50 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: functional weightlessness, bioregenerative life support system,Daphnia, clinorotation, Proteomics |
Contact List
Georg Arnold |
contact affiliation | Gene Centre, LMU Munich |
contact email | arnold@genzentrum.lmu.de |
lab head | |
Kathrin Otte |
contact affiliation | A |
contact email | otte@bio.lmu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002096
- Label: PRIDE project
- Name: The influence of simulated microgravity on the proteome of Daphnia magna