PXD002017
PXD002017 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging |
| Description | Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA,Ting AY. Science, 2013, 339, 1328-1331. doi: 10.1126/science.1230593. Microscopy and mass spectrometry (MS) are complementary techniques: the former provides spatiotemporal information in living cells, but only for a handful of recombinant proteins, while the latter can detect thousands of endogenous proteins simultaneously, but only in lysed samples. Here we introduce technology that combines these strengths by offering spatially- and temporally resolved proteomic maps of endogenous proteins within living cells. The method relies on a genetically-targetable peroxidase enzyme that biotinylates nearby proteins, which are subsequently purified and identified by MS. We used this approach to identify 495 proteins within the human mitochondrial matrix, including 31 not previously linked to mitochondria. The labeling was exceptionally specific and distinguished between inner membrane proteins facing the matrix versus the intermembrane space (IMS). Several proteins previously thought to reside in the IMS or outer membrane, including protoporphyrinogen oxidase, were reassigned to the matrix. The specificity of live-cell peroxidase-mediated proteomic mapping combined with its ease of use offers biologists a powerful tool for understanding the molecular composition of living cells. |
| HostingRepository | MassIVE |
| AnnounceDate | 2015-04-03 |
| AnnouncementXML | Submission_2015-04-03_11:25:06.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Karl Clauser |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification: SILAC-R-0,10-K-0,8; unknown modification: Y-biotin-phenol; unknown modification: C-carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2015-04-03 11:12:03 | ID requested | |
| ⏵ 1 | 2015-04-03 11:25:07 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SILAC, mitochondria, APEX |
Contact List
| Steven A. Carr | |
|---|---|
| lab head | |
| Karl Clauser | |
| contact affiliation | Broad Institute of MIT and Harvard |
| contact email | clauser@broad.mit.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://MSV000079096:a@massive.ucsd.edu |
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