PXD001745
PXD001745 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Automated Absolute and Relative Quantification of SRM Data |
Description | Selected reaction monitoring (SRM) MS is a highly selective and , sensitive technique to quantify protein abundances in complex biological samples. SRM analyses are well supported by state-of-the-art software for both interactive data analysis (e.g., Skyline) and statistical validation of acquired data (e.g., mProphet). However, at present, there is no validated approach to automate the estimation of absolute protein quantities, and manual evaluation, which is widely used, is inherently biased and error-prone. Here, we present Ariadne, a MatlabĀ® software solution supporting automated absolute and relative quantification of SRM data. Ariadne quantifies the targeted peptides listed in the transition lists. If an mProphet output is available for the current experiment, targets can also be filtered according to its statistical validation. Signal processing and statistical learning approaches are combined to provide relative ? and, if requested, absolute ? peptide quantifications. In order to robustly estimate absolute abundances, the external calibration curve method is applied ensuring linearity over the dynamic range. |
HostingRepository | PeptideAtlas |
AnnounceDate | 2015-01-29 |
AnnouncementXML | Submission_2015-01-29_13:03:56.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sandra Goetze |
SpeciesList | scientific name: Drosophila melanogaster; NCBI TaxID: 7227; |
ModificationList | 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine |
Instrument | TSQ Vantage |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2015-01-28 15:26:05 | ID requested | |
⏵ 1 | 2015-01-29 13:03:57 | announced |
Publication List
Unpublished |
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
Sandra Goetze | |
---|---|
contact affiliation | University of Zurich |
contact email | sgoetze@ethz.ch |
dataset submitter | |
Erich Brunner | |
contact affiliation | University of Zurich |
contact email | erich.brunner@imls.uzh.ch |
lab head |
Full Dataset Link List
PeptideAtlas dataset URI |
PASSEL experiment URI |
PASSEL transition group browser URI |