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PXD001745

PXD001745 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAutomated Absolute and Relative Quantification of SRM Data
DescriptionSelected reaction monitoring (SRM) MS is a highly selective and , sensitive technique to quantify protein abundances in complex biological samples. SRM analyses are well supported by state-of-the-art software for both interactive data analysis (e.g., Skyline) and statistical validation of acquired data (e.g., mProphet). However, at present, there is no validated approach to automate the estimation of absolute protein quantities, and manual evaluation, which is widely used, is inherently biased and error-prone. Here, we present Ariadne, a MatlabĀ® software solution supporting automated absolute and relative quantification of SRM data. Ariadne quantifies the targeted peptides listed in the transition lists. If an mProphet output is available for the current experiment, targets can also be filtered according to its statistical validation. Signal processing and statistical learning approaches are combined to provide relative ? and, if requested, absolute ? peptide quantifications. In order to robustly estimate absolute abundances, the external calibration curve method is applied ensuring linearity over the dynamic range.
HostingRepositoryPeptideAtlas
AnnounceDate2015-01-29
AnnouncementXMLSubmission_2015-01-29_13:03:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSandra Goetze
SpeciesList scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine
InstrumentTSQ Vantage
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-01-28 15:26:05ID requested
12015-01-29 13:03:57announced
Publication List
Unpublished
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Sandra Goetze
contact affiliationUniversity of Zurich
contact emailsgoetze@ethz.ch
dataset submitter
Erich Brunner
contact affiliationUniversity of Zurich
contact emailerich.brunner@imls.uzh.ch
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI