PXD001711 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localisation. |
Description | The deubiquitylating enzyme OTUB1 is ubiquitously expressed and is known to target a multitude of substrates, both in the cytosol and nucleus. Here, we demonstrate that phosphorylation of OTUB1 by Casein kinase 2 (CK2) at Ser16 triggers its nuclear accumulation. CK2 phosphorylates OTUB1 at Ser16 in vitro. In cells, endogenous OTUB1 is phosphorylated at Ser16 and this is blocked by chemical and genetic ablation of CK2 activity. Whereas OTUB1 is detected mainly in the cytosol, OTUB1 phosphorylated at Ser16 is detected only in the nucleus. Inhibition of CK2 causes nuclear exclusion of OTUB1. Although phosphorylation of OTUB1 at Ser16 does not alter its catalytic activity, ability to bind K63-linked ubiquitin chains and ability to interact with the E2 enzyme UBE2N in vitro, the nuclear localisation of OTUB1 appears to be essential for IR-induced DNA-damage repair. |
HostingRepository | PRIDE |
AnnounceDate | 2015-04-15 |
AnnouncementXML | Submission_2015-04-15_05:24:29.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gopal Sapkota |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-01-21 01:20:17 | ID requested | |
⏵ 1 | 2015-04-15 05:24:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
curator keyword: Biological |
submitter keyword: OTUB1, Casein kinase, CK2, phosphorylation, ubiquitin, DUB |
Contact List
Gopal P Sapkota |
contact affiliation | MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, Dundee, United Kingdom |
contact email | g.sapkota@dundee.ac.uk |
lab head | |
Gopal Sapkota |
contact affiliation | University of Dundee |
contact email | g.sapkota@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD001711
- Label: PRIDE project
- Name: Casein kinase 2 (CK2) phosphorylates the deubiquitylase OTUB1 at Ser16 to trigger its nuclear localisation.