PXD001634
PXD001634 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | KCMF1 links RAD6 to UBR4 and lysosome-mediated degradation |
| Description | Raw mass spec. data files, converted peak lists and TPP results files for KCMF1 links RAD6 to UBR4 and lysosome-mediated degradation by Hong et. al, MCP 2014. RAD6 is a ubiquitin E2 protein with roles in a number of different biological processes. Here, using affinity purification coupled with mass spectrometry, we identify a number of new RAD6 binding partners, including the E3 ligase KCMF1 (potassium channel modulatory factor 1). NMR, combined with in vivo and in vitro interaction mapping, demonstrate that the KCMF1 C-terminus binds directly to RAD6, while the N-terminus interacts with vesicle- and mitochondria-associated proteins. KCMF1 and RAD6 co-localize at late endosomes and lysosomes, and cells disrupted for KCMF1 or RAD6 function display defects in vesicle dynamics. Notably, we also find that RAD6A point mutants (R7W and R11Q) found in X-linked intellectual disability (XLID) patients specifically lose the interaction with KCMF1, but not with other previously identified RAD6 interactors. We thus identify a new set of RAD6 interacting partners linked to lysosome-mediated degradation, and highlight specific protein-protein interactions that are lost in some RAD6 XLID mutant proteins. |
| HostingRepository | MassIVE |
| AnnounceDate | 2015-01-05 |
| AnnouncementXML | Submission_2015-01-05_13:46:35.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tharan Srikumar |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | deamidated residue; monohydroxylated residue; dihydroxylated residue |
| Instrument | LTQ Orbitrap Velos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2015-01-01 05:14:24 | ID requested | |
| ⏵ 1 | 2015-01-05 13:46:36 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: affinity-purification, AP-MS, FLAG |
Contact List
| Brian Raught | |
|---|---|
| lab head | |
| Tharan Srikumar | |
| contact affiliation | UHN |
| contact email | tharan.srikumar@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://MSV000078983:a@massive.ucsd.edu |
to receive all new ProteomeXchange dataset release announcements!
