PXD001293 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7 |
Description | Macrophages are important immune cells operating at the forefront of innate immunity by taking up foreign particles and microbes through phagocytosis. The RAW 264.7 cell line is commonly used for experiments in the macrophage and phagocytosis field. However, little is known how its functions compare to primary macrophages. Here, we have performed an in-depth proteomics characterisation of phagosomes from RAW 264.7 and bone marrow-derived macrophages by quantifying more than 2500 phagosomal proteins. Our data indicates that there are significant differences for a large number of proteins including important receptors such as mannose receptor 1 and Siglec-1. |
HostingRepository | PRIDE |
AnnounceDate | 2015-02-10 |
AnnouncementXML | Submission_2015-02-10_02:19:01.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matthias Trost |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetic acid derivatized residue; monohydroxylated residue; acetylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-09-08 08:17:17 | ID requested | |
⏵ 1 | 2015-02-10 02:19:02 | announced | |
Publication List
Guo M, H, ä, rtlova A, Dill BD, Prescott AR, Gierli, ń, ski M, Trost M, High-resolution quantitative proteome analysis reveals substantial differences between phagosomes of RAW 264.7 and bone marrow derived macrophages. Proteomics, 15(18):3169-74(2015) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: macrophage, phagosome, mouse, RAW264.7 |
Contact List
Matthias Trost |
contact affiliation | MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, DD1 5EH, Scotland, United Kingdom. |
contact email | m.trost@dundee.ac.uk |
lab head | |
Matthias Trost |
contact affiliation | MRC Protein Phosphorylation Unit |
contact email | m.trost@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001293 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001293
- Label: PRIDE project
- Name: Comaprison of the phagosome proteome of bone marrow-derived macrophages and RAW264.7