PXD001212 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A mammalian transcription factor-specific peptide repository for targeted proteomics |
Description | Site-specific transcription factors (TFs) play an essential role in mammalian development and function as they are vital for the majority of cellular processes. Despite their biological importance, TF proteomic data is scarce in the literature, likely due to difficulties in detecting peptides as the abundance of TFs in cells tends to be low. In recent years, significant improvements in mass spectrometry (MS)-based technologies in terms of sensitivity and specificity have increased the interest in developing quantitative methodologies specifically targeting relatively lowly abundant proteins such as TFs in mammalian models. Such efforts would be greatly aided by the availability of TF peptide-specific information as such data would not only enable improvements in speed and accuracy of protein identifications, but also ameliorate cross-comparisons of quantitative proteomics data and allow for a more efficient development of targeted proteomics assays. However, to date, no comprehensive TF proteotypic peptide database has been developed. To address this evident lack of TF peptide data in public repositories, we are generating a comprehensive, experimentally derived TF proteotypic peptide spectral library dataset based on in vitro protein expression. Our library currently contains peptide information for 89 TFs, and this number is set to increase in the near future. |
HostingRepository | PRIDE |
AnnounceDate | 2015-01-23 |
AnnouncementXML | Submission_2015-01-23_02:03:15.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001212 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Romain Hamelin |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Oxidation; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-08 02:49:50 | ID requested | |
⏵ 1 | 2015-01-23 02:03:16 | announced | |
Publication List
Simicevic J, Moniatte M, Hamelin R, Ahrn, é E, Deplancke B, A mammalian transcription factor-specific peptide repository for targeted proteomics. Proteomics, 15(4):752-6(2015) [pubmed] |
Keyword List
curator keyword: Biological, Highlighted |
submitter keyword: transcription factor , repository for targeted proteomics, quantitative proteomics, Proteotypic peptide selection |
Contact List
Marc Moniatte |
contact affiliation | EPFL Proteomics Core Facility |
contact email | marc.moniatte@epfl.ch |
lab head | |
Romain Hamelin |
contact affiliation | PCF |
contact email | romain.hamelin@epfl.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001212 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001212
- Label: PRIDE project
- Name: A mammalian transcription factor-specific peptide repository for targeted proteomics