PXD001066

PXD001066 is an original dataset announced via ProteomeXchange.

Title	Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation
Description	The differentiation of human CD4+ T cells into different subtypes of T helper cells and regulatory T cells is crucial for an appropriate immunological response. Among all subtypes Th1 cells are the most prominent specific cell type, representing about 50% of all lymphocytes. So far most global proteomic studies have used either only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or applied gel based approaches. These studies have shed light on molecular details of certain aspects of the proteome. Nevertheless a more global analysis of highly pure primary naïve and Th1 cells by LC-MS/MS is needed in order to contribute to the proteome based T cell subtype characterization. We were able to identify 1757 proteins in total, out of which 49 were significantly regulated. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatical comparison to naïve cells reveals the relevance of changes in the metabolism and the ubiquitination pathways upon T cell differentiation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:11:46.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janina Tomm
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-16 02:39:42	ID requested
1	2015-10-13 01:42:20	announced
⏵ 2	2024-10-22 04:11:54	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201400139;

Pagani M, Rockstroh M, Schuster M, Rossetti G, Moro M, Crosti M, Tomm JM, Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation. Proteomics, 15(21):3644-7(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Th1 cells, CD4+,human, LC-MS/MS

Martin von Bergen
contact affiliation	Departments of Proteomics and Metabolomics Helmholtz Centre for environmental research - UFZ Permoserstrasse 15 04318 Leipzig Germany
contact email	martin.vonbergen@ufz.de
lab head
Janina Tomm
contact affiliation	Helmholtzzentrum für Umweltforschung - UFZ
contact email	janina.tomm@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/10/PXD001066

PRIDE project URI

• PRIDE
  1. PXD001066
     1. Label: PRIDE project
     2. Name: Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation