PXD001065

PXD001065 is an original dataset announced via ProteomeXchange.

Title	Compartment resolved reference map from highly purified naive, activated, effector and memory CD8+ cells
Description	Differentiation of CD8+ T lymphocytes into effector and memory cells is key for an adequate immune response and relies on complex interplay of pathways that convey signals from cell surface to nucleus. In this study, we fractionated four CD8+ T cell subtypes; naïve, recently activated effector, effector and memory cells into membrane, cytosol, soluble nucleus, chromatin-bound and cytoskeleton compartments. Using LC-MS/MS analysis, identified peptides were matched to human peptides/proteins (SwissProt). Compartment fractionation and gel-LC-MS separation identified 2399 proteins in total. Among these 735 were detected in all five, 241 in four, 257 in three, 368 in two and 798 found in only one fraction. Comparison between the two most different subsets, naïve and effector, yielded 146 significantly regulated proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:11:55.536.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janina Tomm
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-16 02:39:21	ID requested
1	2015-02-04 04:30:18	announced
⏵ 2	2024-10-22 04:11:56	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201400405;

Zanker D, Otto W, Chen W, von Bergen M, Tomm JM, murine immune cells. Proteomics, 15(11):1808-12(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: murine, CD8+, LC-MS/MS

Martin von Bergen
contact affiliation	Department of Proteomics, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, 04318 Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Janina Tomm
contact affiliation	Helmholtzzentrum für Umweltforschung - UFZ
contact email	janina.tomm@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001065

PRIDE project URI

• PRIDE
  1. PXD001065
     1. Label: PRIDE project
     2. Name: Compartment resolved reference map from highly purified naive, activated, effector and memory CD8+ cells