PXD000976

PXD000976 is an original dataset announced via ProteomeXchange.

Title	Rat Neural Stem Cell Phosphoproteome
Description	Protein phosphorylation is central to the understanding of multiple cellular signaling pathways responsible for regulating the self-renewal and differentiation of neural stem cells (NSCs). Characterization of phosphoproteome is the key to unraveling the underlying mechanism. Here we performed a large-scale phosphoproteomic analysis of rat fetal NSCs (rfNSCs) utilizing strong cation exchange chromatography (SCX) prefractionation and citric acid-assisted two-step enrichment with TiO2 (CATSET) strategy followed by nanoLC-MS/MS analysis. Totally we identified 11,629 phosphosites on 3,046 phosphoproteins, among which 8,146 were class I phosphosites. More than 40% of class I phosphosites were novel when compared with PhosphoSitePlus database. Those phosphorylated proteins were implicated in modulation of diverse nuclear processes, such as transcription regulation, chromatin organization and chromatin modifications. Remarkably, 86 novel phosphosites on 43 transcriptional regulators were identified for the first time, including novel phosphorylation sites on Sox2 (S39) and Sox6 (S439, S442). Furthermore, bioinformatics analysis suggested that a large set of phosphosites were present on proteins involved in diverse pivotal signaling pathways associated with NSCs maintenance, and high degree of phosphorylation in insulin and Wnt signaling pathway was revealed. To our knowledge, this study presents the largest global survey of phosphorylation modification which revealed new regulation network in rfNSCs for the first time and may provide a valuable resource for studying the self-renewal and differentiation of NSCs.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:20:43.302.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuyang Zhao
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-05-15 02:54:27	ID requested
1	2014-05-23 01:17:30	announced
2	2014-07-24 05:18:43	announced	Updated project metadata.
⏵ 3	2024-10-22 04:20:43	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: TiO2, Wnt Signal Pathway,Neural Stem Cell, Phosphoproteome

Jianguo Ji
contact affiliation	State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China
contact email	jijg@pku.edu.cn
lab head
Xuyang Zhao
contact affiliation	Peking University Institute of Systems Biomedicine
contact email	zhao-xu-yang@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD000976
     1. Label: PRIDE project
     2. Name: Rat Neural Stem Cell Phosphoproteome