PXD000959 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | N-linked glycoproteome of human seminal plasma |
Description | N-linked glycosylation is an important type of post-translational modification. This modification plays important roles in many cellular functions including cell-cell and receptor-ligand interactions, immune response, apoptosis, and pathogenesis of many diseases. Seminal plasma is a mixture of secretions from several male accessory glands. Glycoproteins in seminal plasma can reflect the states of reproductive organs or diseases. Using isotope labeling and high-resolution mass spectrometry, we identified 720 N-glycosylated sites on 372 N-glycosylated proteins, corresponding to 365 genes. Analysis of variations among five individuals revealed similar compositions of N-glycosylated proteins in seminal plasma. Consistent composition of N-linked glycoproteins in human seminal plasma provides evidences that seminal plasma glycoproteins are of significant value to be candidates of biomarkers. Bioinformatics annotation of the identified seminal plasma glycoproteins showed that more than 43.0% of these glycoproteins are known secreted proteins according to UniProt. Such a high proportion of secreted glycoproteins in seminal plasma may play important roles in seminal plasma function such as making sure for sperm nutrition and function. |
HostingRepository | PRIDE |
AnnounceDate | 2014-12-15 |
AnnouncementXML | Submission_2014-12-15_08:22:12.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | zhou tao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | (18)O labeled deglycosylated asparagine |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-05-08 03:37:20 | ID requested | |
⏵ 1 | 2014-12-15 08:22:13 | announced | |
Publication List
Yang X, Liu F, Yan Y, Zhou T, Guo Y, Sun G, Zhou Z, Zhang W, Guo X, Sha J, Proteomic analysis of N-glycosylation of human seminal plasma. Proteomics, 15(7):1255-8(2015) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: human,seminal plasma,glycoproteome,LC-MS/MS |
Contact List
Jiahao Sha |
contact affiliation | State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University |
contact email | shajh@njmu.edu.cn |
lab head | |
zhou tao |
contact affiliation | Life Sciences School |
contact email | zhoutao@njmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD000959
- Label: PRIDE project
- Name: N-linked glycoproteome of human seminal plasma