PXD000311

PXD000311 is an original dataset announced via ProteomeXchange.

Title	Analysis of mouse hippocampus membrane proteome by gel-based LC/MS
Description	Mouse hippocampus membrane fractions were prepared using sucrose density gradient ultracentrifugation. Membrane proteins were solubilized using three different condition; 6-ACA/Triton X-100 or ProteoExtract Native Membrane Protein Extraction Kit (Calbiochem, Cat. No. 444810) or ProteoExtract Transmembrane Protein Extraction Kit (Novagen, Cat. No. 71772-3). Solubilized membrane proteins were separated on Blue Native PAGE or SDS-PAGE. Protein bands were excised, destained for peptide sample preparation. Proteins were reduced (DTT), alkylated (iodoacetamide), and in-gel digested with chymotrypsin or trypsin. Digested peptides were extracted then subjected to nanoHPLC and tandem MS analysis with Thermo Orbitrap Velos Pro mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_02:43:10.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000311
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gert Lubec
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: Thermo Orbitrap Velos Pro

Revision	Datetime	Status	ChangeLog Entry
0	2013-07-01 05:24:35	ID requested
1	2014-08-15 02:10:21	announced
2	2014-08-16 00:30:23	announced	Updated project metadata.
⏵ 3	2014-10-23 02:43:11	announced	Updated project metadata.

Heo S, Spoerk S, Birner-Gruenberger R, Lubec G, Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro. Proteomics, 14(17-18):2084-8(2014) [pubmed]

curator keyword: Biological

submitter keyword: Mouse hippocampus, Membrane proteins, Gel-based proteomics, In-gel chymotrypsin/trypsin digestion, LTQ Orbitrap Velos Pro

Gert Lubec
contact affiliation	Department of Pediatrics
contact email	gert.lubec@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD000311

PRIDE project URI

• PRIDE
  1. PXD000311
     1. Label: PRIDE project
     2. Name: Analysis of mouse hippocampus membrane proteome by gel-based LC/MS