PXD000306

PXD000306 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of rainbow trout seminal plasma proteins
Description	In the present study the combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography electrospray ionization tandem mass spectrometry was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins and significantly contributed to the current knowledge regarding the protein composition of fish seminal plasma. The major proteins of rainbow trout seminal plasma, such as transferrin, apolipoproteins, complement C3, serum albumin, hemopexin-like protein, alpha-1-antiproteinase-like and precerebellin-like protein were recognized as acute phase proteins (proteins which plasma concentration changes in response to inflammation). This study provides the basis for further functional studies of fish seminal plasma proteins, as well as for the identification of novel biomarkers for sperm quality.
HostingRepository	PRIDE
AnnounceDate	2014-07-28
AnnouncementXML	Submission_2014-07-28_00:39:51.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD000306
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joanna Nynca
SpeciesList	scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022;
ModificationList	iiodoacetamide derivatized residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	instrument model: LTQ; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2013-06-28 02:52:14	ID requested
1	2014-01-31 07:06:24	announced
⏵ 2	2014-07-28 00:39:52	announced	Updated project metadata.

Nynca J, Arnold GJ, Fr, ö, hlich T, Otte K, Flenkenthaler F, Ciereszko A, Proteomic identification of rainbow trout seminal plasma proteins. Proteomics, 14(1):133-40(2014) [pubmed]

submitter keyword: Rainbow trout, Seminal plasma, LC-MSMS, Proteome

Joanna Nynca
contact affiliation	Polish Academy of Sciences, Department of Gamete and Embryo Biology
contact email	j.nynca@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/01/PXD000306

PRIDE project URI

• PRIDE
  1. PXD000306
     1. Label: PRIDE project
     2. Name: Proteomic identification of rainbow trout seminal plasma proteins