PXD000915

PXD000915 is an original dataset announced via ProteomeXchange.

Title	Effect of Chronic Methamphetamine Exposure on the Neuroproteomics of Rat Brain Tissues
Description	Nowadays, drug abuse and addiction are serious public health problems in the USA. Methamphetamine (METH) is one of the most abused drugs, which is known to cause brain damage from repeated exposure on human. Herein, a proteomic study was applied to evaluate METH-induced brain protein dynamics following a two-week chronic regimen of escalating dose of METH exposure. Proteins were extracted from rat brain hippocampal and olfactory bulb tissues and subjected to liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Both shotgun and targeted proteomic analysis were performed. Protein quantitation was initially based on comparing the spectral counts between METH exposed animals and their control counterparts. Quantitative differences were further confirmed through multiple reaction monitoring (MRM) LC-MS/MS experiments. According to the quantitative results, the expression of 18 proteins (11 in hippocampal proteome, 7 in olfactory bulb proteome) were shown a significant alteration as a result of exposure of rats to METH. 13 of these proteins were up-regulated after METH exposure while 5 of were down-regulated. The altered proteins belonging to different structural and functional families were involved in processes such as cell death, inflammation, oxidation, and apoptosis.
HostingRepository	PRIDE
AnnounceDate	2016-04-21
AnnouncementXML	Submission_2016-04-21_04:52:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rui Zhu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-04-22 01:28:35	ID requested
⏵ 1	2016-04-21 04:52:19	announced

Zhu R, Yang T, Kobeissy F, Mouhieddine TH, Raad M, Nokkari A, Gold MS, Wang KK, Mechref Y, The Effect of Chronic Methamphetamine Exposure on the Hippocampal and Olfactory Bulb Neuroproteomes of Rats. PLoS One, 11(4):e0151034(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Proteomics, methamphetamine, escalating dose, neuroproteomics, systems biology, drug abuse, chronic administration

Yehia Mechref
contact affiliation	Texas Tech University
contact email	yehia.mechref@ttu.edu
lab head
Rui Zhu
contact affiliation	Texas Tech University
contact email	rui.zhu@ttu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/04/PXD000915

PRIDE project URI

• PRIDE
  1. PXD000915
     1. Label: PRIDE project
     2. Name: Effect of Chronic Methamphetamine Exposure on the Neuroproteomics of Rat Brain Tissues