PXD006579

PXD006579 is an original dataset announced via ProteomeXchange.

Title	ISDoT: In Situ Decellularization of Tissues for proteomic analysis of native extracellular matrix
Description	The extracellular matrix (ECM) is a master regulator of cellular phenotype and behavior. It plays a critical role in both normal tissue homeostasis and disease pathology. We have developed a fast and efficient approach to enhance the study of ECM composition and structure. Termed In Situ Decellularization of Tissues (ISDoT), it allows whole organs to be decellularized leaving native ECM architecture intact. We demonstrate how our approach enriches for ECM components allowing deep quantitative proteomic interrogation of the ECM. Using the ISDoT procedure, native ECM under normal and pathological conditions can be catalogued in unprecedented detail.
HostingRepository	PRIDE
AnnounceDate	2017-06-12
AnnouncementXML	Submission_2018-07-17_08:17:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Cox
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-hydroxyproline; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-05-24 04:52:31	ID requested
1	2017-06-12 01:35:31	announced
⏵ 2	2018-07-17 08:17:48	announced	Updated publication reference for PubMed record(s): 29964200.

Socovich AM, Naba A, The cancer matrisome: From comprehensive characterization to biomarker discovery. Semin Cell Dev Biol, 89():157-166(2019) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Technical, Biomedical

submitter keyword: Biomedical, Cancer, Extracellular Matrix, Decellularisation, ISDoT

Janine Erler
contact affiliation	Biotech Research and Innovation Centre (BRIC) University of Copenhagen, Copenhagen, Denmark
contact email	janineerler@bric.ku.dk
lab head
Thomas Cox
contact affiliation	Garvan Institute of Medical Research, The Kinghorn Cancer Centre, Sydney, Australia
contact email	t.cox@garvan.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006579

PRIDE project URI

• PRIDE
  1. PXD006579
     1. Label: PRIDE project
     2. Name: ISDoT: In Situ Decellularization of Tissues for proteomic analysis of native extracellular matrix