<<< Full experiment listing

RPXD034731

RPXD034731 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	JPST000100-2 B. subtilis phosphoproteomics [Reanalysis: JPST000100]
Description	Protein phosphorylation is a crucial posttranslational modification for regulating cellular processes in bacteria; however, it has not been extensively studied because of technical difficulties in the enrichment of phosphopeptides. We devised an enrichment protocol that enabled the identification of >1000 phosphopeptides from a single bacterial sample. We discovered three high-confidence serine and threonine phosphorylation motifs, as well as 29 other motifs at various levels of confidence, from three distinct bacterial phosphoproteomes. We found that the proline-directed and basophilic phosphorylation motifs that are commonly enriched in eukaryotes were not observed in bacteria. Unlike eukaryotes, bacteria had a low occurrence of both phosphorylation and acetylation in N-terminal phosphopeptides. Because infection of host cells by bacterial pathogens is often accompanied by kinase-mediated phosphorylation events, the differences in phosphorylation preferences between bacteria and eukaryotes revealed by this study could be useful in identifying bacterial-specific targets for future therapies. [Original project description]
HostingRepository	jPOST
AnnounceDate	2023-03-25
AnnouncementXML	Submission_2023-03-25_06:06:33.785.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD034731
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000100
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Bacillus subtilis; NCBI TaxID: 1423;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; phosphorylated L-histidine; L-aspartic 4-phosphoric anhydride; alpha-amino acetylated residue
Instrument	Q Exactive

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2022-06-20 17:56:06	ID requested
0	1	2023-03-25 06:06:34	announced	JPST000100-2 B. subtilis phosphoproteomics [Reanalysis: JPST000100]

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis, bacteria
E.coli
phosphorylation

submitter keyword: reanalysis, bacteria

E.coli

phosphorylation

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001730/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001730/