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RPXD034723

RPXD034723 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary
TitleJPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096]
DescriptionCatecholamines promote lipolysis both in brown and white adipocytes, whereas the same stimuli preferentially activate thermogenesis in brown adipocytes. Molecular mechanisms for the adipose-selective activation of thermogenesis remain poorly understood. Here, we employed quantitative phosphoproteomics to map global and temporal phosphorylation profiles in brown, beige, and white adipocytes under β3-adrenenoceptor activation and identified kinases responsible for the adipose-selective phosphorylation profiles. We found that casein kinase2 (CK2) activity is preferentially higher in white adipocytes than brown/beige adipocytes. Genetic or pharmacological blockade of CK2 in white adipocytes activates the thermogenic program in response to cAMP stimuli. Such activation is largely through reduced CK2-mediated phosphorylation of class I HDACs. Notably, inhibition of CK2 promotes beige adipocyte biogenesis and leads to an increase in whole-body energy expenditure and ameliorates diet-induced obesity and insulin resistance. These results indicate that CK2 is a plausible target to rewire the β3-adrenenoceptor signaling cascade that promotes thermogenesis in adipocytes. [Original project description]
HostingRepositoryjPOST
AnnounceDate2023-03-11
AnnouncementXMLSubmission_2023-03-21_03:44:39.860.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/RPXD034723
ReviewLevelNon peer-reviewed dataset
DatasetOriginReanalysis of JPST000096
RepositorySupportSupported dataset by repository
PrimarySubmitterjPOST reanalysis
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentQ Exactive
Dataset History
ReanalysisRevisionDatetimeStatusTitle / ChangeLog Entry
02022-06-20 17:55:19ID requested
012023-03-11 06:53:50announcedJPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096]
022023-03-21 03:44:40announcedJPST000096-1 Beige adipocyte phosphoproteomics [Reanalysis: JPST000096]
2023-03-21: Updated AnnounceDate.
Publication List
no publication
Keyword List
submitter keyword: reanalysis, Beige adipocyte, phosphoproteomics
Contact List
Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliationjPOST
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001720/