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RPXD034323

RPXD034323 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	JPST000068 monolith HILIC-HeLa [Reanalysis: JPST000068]
Description	A meter-scale monolithic silica capillary column modified with urea-functional groups for hydrophilic interaction liquid chromatography (HILIC) was developed for highly efficient separation of biological compounds. We prepared a ureidopropylsilylated monolithic silica capillary column with a minimum plate height of 12 Î¼m for nucleosides and a permeability of 2.1 Ã— 10âˆ’13 m2, which is comparable with the parameters of monolithic silica-C18 capillary columns. Over 300,000 theoretical plates were experimentally obtained in HILIC with a 4 m long column at 8 MPa; this is the best result yet reported for HILIC. A 2 m long ureidopropylsilylated monolithic silica capillary column was utilized to develop a HILIC mode LC-MS system for proteomics applications. Using tryptic peptides from human HeLa cell lysate proteins, we identified the comparable numbers of peptides and proteins in HILIC with those in reversed-phase liquid chromatography (RPLC) using a C18-modified monolithic silica column when shallow gradients were applied. In addition, approximately 5-fold increase in the peak response on average was observed in HILIC for commonly identified tryptic peptides due to the high acetonitrile concentration in the HILIC mobile phase. Since HILIC mode LC-MS shows orthogonal selectivity to RPLC mode LC-MS, it is useful as a complementary tool to increase proteome coverage in proteomics studies. [Original project description]
HostingRepository	jPOST
AnnounceDate	2023-04-01
AnnouncementXML	Submission_2023-04-01_00:33:04.926.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD034323
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000068
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	TripleTOF 5600

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2022-06-05 06:19:54	ID requested
0	1	2023-04-01 00:33:05	announced	JPST000068 monolith HILIC-HeLa [Reanalysis: JPST000068]

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis, monolithic silica column, HILIC

submitter keyword: reanalysis, monolithic silica column, HILIC

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001653/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001653/