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RPXD018479

RPXD018479 is container for one or more analyses. The general container metadata is provided below and the table under Dataset History provides links to the various reanalyses have been provided (if any) in this container.

Reanalysis Container Summary

Title	Reanalysis dataset of JPST000206
Description	Quantitative phosphoproteome analyses of colorectal cancer cell lines (HCT116 metastatic clone and the parent clone without metastasis) were carried out using IMAC-based posphopeptide enrichment followed by SCX-RP-MS/MS with iTRAQ labeling(metastasis:iTRAQ116 or 117, no metastasis: iTRAQ114 or 115). For this reanalysis, MS Datasets were processed for peak picking with MaxQuant v1.6.2.10, and the generated peak lists were searched against human entries of UniProt/Swiss-Prot release 2018_10 using three search engines: MaxQuant v1.6.2.10, X!Tandem v2015.04.01.1, and Comet 2015.02 rev. 0. The decoy database was generated by reversing sequences from the database. Then, the sum of the total number of matched b/y ions and the total number of amino acids comprising the peptide sequence tag were counted to score the reliability of peptide identification independent of these search engines. Finally, the 1% false discovery rate was controlled by the target-decoy approach with this score.
HostingRepository	jPOST
AnnounceDate	2020-05-27
AnnouncementXML	Submission_2022-09-18_03:53:59.725.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/RPXD018479
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of JPST000206
RepositorySupport	Supported dataset by repository
PrimarySubmitter	jPOST reanalysis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; iTRAQ4plex reporter+balance reagent N-acylated residue; iTRAQ4plex reporter+balance reagent acylated N-terminal; iTRAQ4plex reporter+balance reagent O-acylated residue; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Velos

Dataset History

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2020-04-12 23:23:33	ID requested
0	1	2020-05-26 23:36:09	announced	Reanalysis dataset of JPST000206
0	2	2021-02-04 16:47:46	announced	Reanalysis dataset of JPST000206 2021-02-04: Updated description and FTP location.
0	3	2022-09-18 03:54:00	announced	Reanalysis dataset of JPST000206 2022-09-18: Updated FTP location.

Publication List

no publication

no publication

Keyword List

submitter keyword: reanalysis

submitter keyword: reanalysis

Contact List

Yasushi Ishihama
lab head
jPOST reanalysis
contact affiliation	jPOST
contact email	reanalysis@jpostdb.org
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000805/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000805/