RPXD000669

RPXD000669 is an old-style reanalysis announcement missing a container. This dataset should be updated with a proper container and revision following 2019 ProteomeXchange protocols.

Title	Reprocessed: Proteomic and phosphoproteomic data of colorectal cancer tissues and cells for Chromosome-Centric Human Proteome Project
Description	Protein extraction and proteolytic digestion were performed using a Filter-Assisted Sample Preparation (FASP) protocol. In case of phosphopeptide enrichment immobilized Fe(III) affinity chromatography (Fe-IMAC) was performed. The peptides were fractionated using an HPLC system fitted with an SCX column. 10 sample datasets were used: 1. CRC_N: Normal tissue samples were obtained from 20 colorectal cancer patients, no quantification.Phosphopeptide enrichment was performed. 2. CRC_T: Human colorectal cancer tissue samples were obtained from 20 patients, no quantification. Phosphopeptide enrichment was performed. 3. CRC_iTRAQ: Human colorectal cancer tissue samples were obtained from 24 patients, iTRAQ quantification. 4. CRC_phospho_iTRAQ:Human colorectal cancer tissue samples were obtained from 24 patients, iTRAQ quantification.Phosphopeptide enrichment was performed. 5. HCT116_iTRAQ: Human colon cancer cell HCT116, iTRAQ quantification. 6. HCT116_phospho_iTRAQ: Human colon cancer cell HCT116, phosphopeptide enrichment, iTRAQ quantification. 7. SW_SILAC_HL: Human colon cancer cell SW480 and SW620, SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW480; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW620; heavy). 8. SW_SILAC_LH: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW620; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW480; heavy). 9. SW_phospho_SILAC_HL: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW480; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW620; heavy). Phosphopeptide enrichment was performed. 10. SW_phospho_SILAC_LH: SW480 and SW620 were grown in SILAC media, containing l-arginine (Arg0) and l-lysine (Lys0) (SW620; light), or 13C615N4-l-arginine (Arg10) and 13C6-l-Lysine (Lys6) (SW480; heavy). Phosphopeptide enrichment was performed. Reprocessed by PeptideAtlas;
HostingRepository	PeptideAtlas
AnnounceDate	2013-12-23
AnnouncementXML	Submission_2013-12-23_14:04:13.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Reanalysis of PXD000089
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zhi Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C,4x(15)N labeled L-arginine; iTRAQ4plex-114 reporter+balance reagent acylated residue; S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; 6x(13)C labeled L-lysine
Instrument	LTQ Orbitrap Velos

Reanalysis	Revision	Datetime	Status	Title / ChangeLog Entry
	0	2013-12-23 12:59:37	ID requested
	1	2013-12-23 14:00:17	announced	Reprocessed: Proteomic and phosphoproteomic data of colorectal cancer tissues and cells for Chromosome-Centric Human Proteome Project
	2	2013-12-23 14:04:32	announced	Reprocessed: Proteomic and phosphoproteomic data of colorectal cancer tissues and cells for Chromosome-Centric Human Proteome Project Add one more PeptideAtlas dataset URI

no publication

curator keyword: Human colorectal cancer tissues, Human colon normal tissues, phosphopeptides, iTRAQ,

Zhi Sun
contact affiliation	Institute for Systems Biology
contact email	zhi.sun@systemsbiology.org
dataset submitter
Eric Deutsch
contact affiliation	Institute for Systems Biology
contact email	edeutsch@systemsbiology.org
lab head

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