PXD083942

PXD083942 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of Amphioctopus fangsiao sperm reveals molecular adaptations to long-term storage
Description	Long-term sperm storage in the female spermatheca allows Amphioctopus fangsiao to separate mating from fertilization, but the molecular responses of stored sperm remain unclear. We compared female-stored sperm (FS) with male sperm (MS) using Data-independent acquisition (DIA)-based quantitative proteomics and measured lactate content and mitochondrial membrane potential. A total of 2,169 differentially abundant proteins were identified, including 1,890 proteins with higher abundance and 279 with lower abundance in FS relative to MS. GO analysis identified 54 significantly enriched terms, including 37 related to sperm storage, while KEGG analysis identified 17 enriched pathways, including 8 associated with energy metabolism, stress responses, cellular survival, and reproductive signaling. PPI analysis of 868 well-annotated proteins identified 12 key proteins involved in energy metabolism, proteostasis, stress regulation, receptor-linked signaling, and calcium regulation. ENO1, PGM1, and PHGDH showed higher abundance in FS, whereas lactate content and mitochondrial membrane potential did not differ significantly between FS and MS. Lactate levels differed significantly between sperm and gill tissues. These findings indicate that long-term sperm storage involves coordinated proteomic remodeling that maintains metabolic flexibility, stress tolerance, and cellular stability while preserving sperm viability.
HostingRepository	iProX
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_18:49:09.632.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiantao Yao
SpeciesList	scientific name: Amphioctopus fangsiao; NCBI TaxID: 515817;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-09 18:48:45	ID requested
⏵ 1	2026-09-09 18:49:10	announced

Dataset with its publication pending

submitter keyword: Amphioctopus fangsiao, Proteomic analysis, Sperm storage, Data-independent acquisition

Yanwei Feng
contact affiliation	School of Fisheries, Ludong University
contact email	fywzxm1228@163.com
lab head
Jiantao Yao
contact affiliation	School of Fisheries, Ludong University
contact email	3421614981@qq.com
dataset submitter

iProX dataset URI