PXD083937

PXD083937 is an original dataset announced via ProteomeXchange.

Title	Efficient Capture and Proteomic Profiling of Extracellular Vesicles with Magnetic Cilia Channel Chip for Prostate Cancer Prognosis
Description	Extracellular vesicles (EVs) are promising non-invasive biomarkers, but their clinical application is limited by inefficient isolation and downstream analysis. In this study, we developed a biomimetic magnetic cilia channel chip (MagCilia-chip) for the efficient capture and proteomic profiling of urinary EVs. Tim4-functionalized magnetic microcilia specifically captured phosphatidylserine-positive EVs, while magnetic-field-driven three-dimensional rotation enhanced EV–microstructure interactions and enabled on-chip EV lysis and enzymatic digestion. Data-independent acquisition mass spectrometry identified 2,903 EV-derived proteins from only 50 μL of urine. Comparative proteomic profiling was performed using an independent discovery cohort comprising 12 patients with prostate cancer and 8 post-treatment patients, resulting in the identification of 368 differentially abundant EV proteins. An independent parallel reaction monitoring cohort comprising 19 prostate cancer and 9 post-treatment samples was used for targeted validation. Differential abundance of C9, COL6A3, and VTN was confirmed, and the resulting three-protein candidate panel was evaluated using multiple machine-learning classifiers. This dataset supports the development of urinary EV-based proteomic approaches for prostate cancer research and post-treatment monitoring.
HostingRepository	iProX
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_18:43:02.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mingyang Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized glutamic acid; oxidation
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-09 18:42:40	ID requested
⏵ 1	2026-09-09 18:43:03	announced

Dataset with its publication pending

submitter keyword: Human, Urine, Extracellular vesicles, Prostate cancer, Proteomics, DIA-MS, PRM

Zhuoying Xie
contact affiliation	Southeast University
contact email	zyxie@seu.edu.cn
lab head
Mingyang Li
contact affiliation	Southeast University
contact email	230229239@seu.edu.cn
dataset submitter

iProX dataset URI