PXD083885

PXD083885 is an original dataset announced via ProteomeXchange.

Title	Nuclear TRiC states captured in situ
Description	The chaperonin T-complex protein ring complex (TRiC) is an essential protein-folding machine in eukaryotic cells. Although its cytosolic role in folding newly synthesized proteins is well established, nuclear TRiC remains less well described. Here, we combined cryo-electron tomography (cryo-ET), cross-linking and affinity purification mass spectrometry with AlphaFold3 (AF3)-based structural modeling to examine nuclear TRiC in human cells. We detected intermediates of the TRiC duty cycle in the nucleus and identified several nuclear proteins that bind to TRiC. The respective AF3 models suggest interaction surfaces at the inside of the TRiC chamber. Our findings point towards compartment-specific roles of TRiC and a potential function in maintaining nuclear proteostasis. By data mining very large datasets, our study establishes that rather low-abundant events can be analyzed by cryo-ET. It thus paves the way towards broader applications.
HostingRepository	PRIDE
AnnounceDate	2026-09-09
AnnouncementXML	Submission_2026-09-09_14:16:09.132.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-09 07:08:39	ID requested
⏵ 1	2026-09-09 14:16:09	announced

Dataset with its publication pending

submitter keyword: AP-MS, cryo-ET,TRiC complex

Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research Frankfurt am Main Germany
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD083885

PRIDE project URI

• PRIDE
  1. PXD083885
     1. Label: PRIDE project
     2. Name: Nuclear TRiC states captured in situ