PXD083764

PXD083764 is an original dataset announced via ProteomeXchange.

Title	PGM3-Driven Hexosamine Biosynthesis Facilitates Hepatocellular Carcinoma Progression through YBX1 O-GlcNAcylation (Glycoproteomics)
Description	Phosphoglucomutase 3 (PGM3) is one of the rate-limiting enzymes in the hexosamine biosynthesis pathway (HBP) and a key regulator of HBP metabolic flux. Despite the functional role of PGM3 has been validated in various tumors, its function and molecular mechanism in hepatocellular carcinoma (HCC) remain to be elucidated. This study found that PGM3 expression is upregulated in HCC tissues, and high PGM3 expression is closely associated with poor prognosis in HCC patients. In vitro and in vivo results demonstrated that PGM3 enhances UDP-GlcNAc synthesis and protein O-GlcNAcylation, thereby promoting HCC proliferation, migration, and invasion. Further O-GlcNAcylomic sequencing of HCC cells identified Y-box binding protein 1 (YBX1) as a key substrate for PGM3-mediated O-GlcNAcylation. Notably, O-GlcNAcylation of YBX1 at a specific serine residue (Ser165) enhances its nuclear translocation. Additionally, we validated that FR054, a novel reported PGM3 inhibitor, retards HCC progression by diminishing PGM3-mediated O-GlcNAcylation. These interventions effectively inhibit HCC malignant progression. Collectively, our findings reveal the potential therapeutic value of targeting the PGM3-HBP-YBX1 signaling axis in HCC treatment.
HostingRepository	iProX
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-06_20:12:10.162.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiaqing Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-06 20:11:03	ID requested
⏵ 1	2026-09-06 20:12:10	announced

Dataset with its publication pending

submitter keyword: Hepatocellular carcinoma, Hexosamine biosynthesis pathway, PGM3, O-GlcNAcylation, YBX1, FR054

Beicheng Sun
contact affiliation	The First Affiliated Hospital of Anhui Medical University
contact email	sunbc@ahmu.edu.cn
lab head
Jiaqing Chen
contact affiliation	The First Affiliated Hospital of Anhui Medical University
contact email	2345011394@stu.ahmu.edu.cn
dataset submitter

iProX dataset URI