PXD083530

PXD083530 is an original dataset announced via ProteomeXchange.

Title	Extending on-antibody TMT labeling to lysine acylations enables dynamic acetyl-proteomic profiling
Description	We developed an on-antibody tandem mass tag (TMT) labeling workflow for quantitative profiling of multiple lysine acylations and validated its reliability in Escherichia coli and Saccharomyces cerevisiae. The method enabled reproducible analysis of acetylation, propionylation, malonylation, and succinylation. Using this platform, we characterized dynamic acetylome changes in E. coli under temperature and pH perturbations, growth-cycle progression, and carbon-source alterations. We further identified AceA K308 and Ahr K127 as functional acetylation sites, with mutations reducing enzyme activity. This workflow provides a robust tool for studying lysine acylation and metabolic adaptation.
HostingRepository	iProX
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-09-02_00:29:54.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinglong Jia
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-09-02 00:29:33	ID requested
⏵ 1	2026-09-02 00:29:55	announced

Dataset with its publication pending

submitter keyword: On-antibody TMT labeling

Minjia Tan
contact affiliation	Shanghai institute of Materia Medica Chinese Academy of Sciences
contact email	Tanminjia@outlook.com
lab head
Xinglong Jia
contact affiliation	Shanghai institute of Materia Medica Chinese Academy of Sciences
contact email	jiaxlzzu@163.com
dataset submitter

iProX dataset URI