⮝ Full datasets listing
PXD083249
PXD083249 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | GIGYF2 regulates telomere length and cellular senescence by restricting telomerase access |
| Description | This dataset presents the proximity-dependent biotinylation (PhastID) proteomic profiling of GIGYF2, a newly identified negative regulator of telomerase. Human cells expressing BirA-fused GIGYF2 were labeled with 50 μM biotin for 4 hours. Biotinylated protein complexes were captured using streptavidin magnetic beads, followed by on-bead reduction, alkylation, and tryptic digestion. The resultant peptides were analyzed by LC-MS/MS on an Orbitrap Fusion Lumos instrument. Data processing was performed using MaxQuant (v2.1.0.0) against the UniProt human database. Protein abundance was normalized by calculating Fraction of Target (FOT) values based on iBAQ intensities. This interactome analysis reveals GIGYF2's association with the CCR4-NOT complex and the shelterin components TPP1/POT1, supporting its role in post-transcriptional regulation and telomere homeostasis. This resource contributes to the mechanistic understanding of telomere biology and potential therapeutic targeting in aging. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-26 |
| AnnouncementXML | Submission_2026-08-26_18:26:35.539.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chuanle Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-26 18:25:20 | ID requested | |
| ⏵ 1 | 2026-08-26 18:26:36 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: GIGYF2, PhastID, networks |
Contact List
| Yan Huang | |
|---|---|
| contact affiliation | School of Lifesciences, Sun Yat-sen University |
| contact email | huangy336@mail.sysu.edu.cn |
| lab head | |
| Chuanle Wang | |
| contact affiliation | School of Lifesciences, Sun Yat-sen University |
| contact email | 1415849898@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
