⮝ Full datasets listing
PXD083164
PXD083164 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stiffness Programs WDR44 Phosphorylation to Gate Cargo Sorting into Migrasomes |
| Description | Cells release extracellular vesicles (EVs) for intercellular communication, homeostasis, and microenvironment remodeling, yet how physiological matrix stiffness controls EV cargo sorting remains unclear. Here we show that stiffness programs a cargo-selective sorting pathway in migrasomes, where matrix stiffness couples Integrin β1–FAK–AKT to AKT-dependent phosphorylation of WDR44. Critically, phosphorylated WDR44 acts as a molecular gate that enables Rab11b-dependent trafficking of intraluminal nanovesicles via Myosin Va into migrasomes. Consistently, physiologically stiffness-programmed migrasomes enriched in CSF1 enhance macrophage-recruiting transport and accelerate wound closure in vitro. Together, these findings reveal a mechanistic framework for stiffness-regulated migrasome cargo sorting, and the tunable GA hydrogel platform (300–2,300 Pa) provides a scalable way to guide EV production under defined mechanical conditions. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-24 |
| AnnouncementXML | Submission_2026-08-25_18:19:06.905.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenmin Tian |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-25 18:18:48 | ID requested | |
| ⏵ 1 | 2026-08-25 18:19:07 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Extracellular vesicles (EVs),WDR44,Matrix stiffness,AKT-dependent phosphorylation,Rab11b |
Contact List
| Yang Chen | |
|---|---|
| contact affiliation | The Center for Precision Medicine Multi-omics Research (CPMMR) |
| contact email | chenyang1816185048@bjmu.edu.cn |
| lab head | |
| Wenmin Tian | |
| contact affiliation | The Center for Precision Medicine Multi-omics Research (CPMMR) |
| contact email | tianwenmin2019@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
