⮝ Full datasets listing
PXD083134
PXD083134 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of the proteome of P. aeruginosa under different combinations of cAMP and c-di-GMP levels |
| Description | The aim of this experiment was to assess proteomic changes in P. aeruginosa PAO1 under different combinations of intracellular cAMP and c-di-GMP levels. Intracellular c-di-GMP levels were manipulated by expressing the P. aeruginosa phosphodiesterase PA5295 (LG) or the diguanylate cyclase WspR19 (HG) from pBBR5-MCS. The empty plasmid served as the unaltered c-di-GMP condition (FG). Intracellular cAMP signaling was reduced by deletion of the adenylate cyclase genes cyaA and cyaB (LA), or increased by deletion of the cAMP phosphodiesterase gene cpdA (HA). The parental background served as the unaltered cAMP control (FA). Each cAMP genotype was combined with each c-di-GMP genotype, yielding a nine-state signaling toolbox. Cultures were grown overnight at 37 C with agitation in MOPS based minimal medium supplemented with either glucose and NH4+ (-G-N) or glucose and choline (-G-C). |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_23:48:12.839.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Thomas Bock |
| SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-25 07:14:38 | ID requested | |
| ⏵ 1 | 2026-08-25 23:48:13 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: cAMP, P. aeruginosa, DatasetType:Proteomics |
Contact List
| Alexander Schmidt | |
|---|---|
| contact affiliation | Biozentrum, Universtiy of Basel, 4056 Basel, Switzerland |
| contact email | alex.schmidt@unibas.ch |
| lab head | |
| Thomas Bock | |
| contact affiliation | Uni Basel |
| contact email | thomas.bock@unibas.ch |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v14/MSV000103009/ |
