⮝ Full datasets listing
PXD083040
PXD083040 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TRRAP enables MMB-dependent mitotic transcription and determines sensitivity to CHK1 inhibition |
| Description | Precise temporal activation of mitotic genes is essential for proper cell-cycle progression, yet the chromatin-based mechanisms coordinating this process remain incompletely understood. The B-MYB-MuvB (MMB) complex is a key regulator of mitotic gene expression and becomes hyperactivated following inhibition of the ATR-CHK1 pathway, resulting in MMB-dependent premature mitotic entry, replication catastrophe, and cell death. Here, we combine proximity-dependent proteomics with a targeted siRNA screen of chromatin regulators using CHK1 inhibitor-induced premature mitosis and DNA damage as functional readouts. We identify TRRAP as a critical regulator of MMB-dependent mitotic transcription. TRRAP physically associates with B-MYB, occupies MMB target promoters, and is required for expression of mitotic genes during both normal G2/M progression and CHK1 inhibitor-induced premature mitosis. Although activation of YAP signaling bypasses the G1 requirement for TRRAP, TRRAP remains essential for G2/M gene expression and mitotic progression. Together, these findings link TRRAP-dependent chromatin regulation to MMB activity and checkpoint sensitivity. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_23:44:27.955.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Leibniz FLI Proteomics |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Carbamidomethyl; Acetyl; Biotin |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-24 00:27:48 | ID requested | |
| ⏵ 1 | 2026-08-25 23:44:28 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: TRRAP, B-MYB, MuvB, mitotic transcription, ATR-CHK1 pathway, YAP, DatasetType:Proteomics |
Contact List
| Bjoern von Eyss | |
|---|---|
| contact affiliation | Leibniz Institute on Aging, Fritz Lipmann Institute, Beutenbergstrasse 11 07745 Jena, |
| contact email | Bjoern.vonEyss@leibniz-fli.de |
| lab head | |
| Leibniz FLI Proteomics | |
| contact affiliation | Leibniz FLI |
| contact email | proteomics@leibniz-fli.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v14/MSV000102991/ |
